LIPOFECTAMINE LTX PROTOCOL PDF

Lipofectamine® LTX Reagent offers a streamlined protocol—no need to remove transfection complexes or change/add medium following transfection. A simple. Lipofectamine LTX® Reagent is a proprietary, animal-origin free formulation for the or contact Technical Services for other specialized transfection protocols. protocol applicable to Invitrogen products, as set forth below (the “Protocol”). by adding 50 μL of Lipofectamine™ LTX to μL of Opti-MEM® medium.

Lipofectamien, we describe the comparison of transfection of HUVEC using nine chemical transfection reagents, currently commercially available, to identify the reagent that elicits the highest transfection efficiency without compromising cell viability.

Identification by morphologic and immunologic criteria. Articles from Journal of Biomolecular Techniques: We thank Sigma-Aldrich, Fermentas, and Roche, which kindly supplied free samples of their transfection reagents for us to test.

All protocols are per-well of a six-well plate. Journal List J Biomol Tech v.

Modification of adenovirus gene transfer vectors with synthetic polymers: Toward development of artificial viruses for gene therapy: Biotechnol Prog ; The nine compounds tested in this study included activated dendrimers, cationic polymers of linear PEI, lipids and polyamines, nonliposomal lipids, polycationic lipids, and cationic lipids, reflecting the broad categories of chemical transfection reagents available and the intense development in this area.

Inhibition of hydrophobic protein-mediated Candida albicans attachment to endothelial cells during physiologic shear flow. Cytokines Mol Ther ; 2: Chemical transfection reagents have been shown to reduce growth and viability of cells after transfection, possibly as a result of changes in the strength of the cell membrane.

siRNA transfection in endothelial cells – siRNA, microRNA and RNAi

Currie1 Bridget A. Curr Drug Saf ; 3: Reporter gene expression for monitoring gene transfer. Azzam T, Domb AJ. J Cell Sci ; Bioconjug Chem ; Hum Gene Ther ; Mol Ther ; Br J Haematol ; Mixtures were incubated for 5 min and then combined together for a further 20 min. Other studies have reported differences in cell characteristics between HUVEC from single or multiple-pooled donors, 35 which may explain this variability.

Identification of replicative senescence-associated genes in human umbilical vein endothelial cells by an annealing control primer system. Primary cells, such as HUVEC, are notoriously difficult to transfect and are susceptible to the toxic effects of transfection reagents. This study analyzed nine currently available, commercial transfection reagents and showed that cationic lipid reagents were the most efficient in gene-modifying HUVEC.

An electroporation protocol for efficient DNA transfection in PC12 cells.

Towards endothelial-cell-directed cancer immunotherapy: Mol Ther ; 5: Improving safety of gene therapy. Importantly, dead cells tend to detach from the growth surface and thus, were not analyzed in this study. Dual targeting of gene delivery by genetic modification of adenovirus serotype 5 fibers and cell-selective transcriptional control. Our study demonstrated that a small selection of commercially available chemical transfection reagents was able to transfer exogenous genes efficiently to primary human cells.

A number of reasons have been considered to explain these differences. These reagents show promise, as they are produced easily in large quantities, are used rapidly in high-throughput assays, are noninfectious, and can transfer DNA of various sizes.

Regulatory considerations for novel gene therapy products: