Interlaboratory testing of methods for assay of xylanase activity. Bailey M.J.; Biely P.; Poutanen K. Journal of Biotechnology 23(3): Abstract Twenty laboratories participated in a collaborative investigation of assays for endo-1,4-β-xylanase activity based on production of reducing sugars from. See Bailey, M.J., Biely, P., and Poutanen, K., Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, p. ).
|Published (Last):||11 July 2007|
|PDF File Size:||20.99 Mb|
|ePub File Size:||5.76 Mb|
|Price:||Free* [*Free Regsitration Required]|
Interlaboratory testing of methods for assay of xylanase activity.
PDF of Interlaboratory testing of methods for assay of xylanase activity
View in Source Cite this paper. The substrates and methods already in use in the different laboratories were first recorded and the apparent activities obtained using these methods in the analysis of a distributed enzyme sample were compared. Significant reduction in interlaboratory variation was obtained when all the participants used the same substrate for activity determination, each with their own assay procedure.
The level of agreement was further improved when both the substrate and the method procedure were standardized. This figure probably reflects the inherently poor reproducibility of results when using only partially soluble, poorly defined and rather impure polymeric substrates.
The fot level of variation was however low enough to allow meaningful comparison of results obtained in different laboratories when using the standardized assay substrate and method procedure. Fifteen laboratories also participated in preliminary testing of an assay based on the release of dyed fragments tesying 4- O -methyl glucuronoxylan dyed with Remazol Brilliant Blue dye.
High values of the coefficients of correlation indicated good linearity between the amount of dyed fragments released and enzyme concentration. Standard deviation Chromatography Biochemistry Quantitative analysis chemistry Substrate chemistry Round robin test Chemistry Xylanase. Norton Nelson 7 Estimated H-index: Sumner 5 Estimated H-index: Michael Somogyi 17 Estimated H-index: Measurement of xylanase activity with insoluble xylan substrate.
M Nummi 3 Estimated H-index: Interlaborahory of xylanolytic enzymes by strains of Aspergillus. Bailey 3 Estimated H-index: Miller 1 Estimated H-index: Measurement of carboxymethylcellulase activity. Gail Lorenz Miller 6 Estimated H-index: Non-Corrosive dye reagent for detection of reducing sugars in borate complex methodw chromatography.
Sinner 1 Estimated H-index: A note on the use of dinitrosalicylic acid for determining the products of enzymatic reactions. Thermostable Enzymes in Lignocellulose Hydrolysis. Liisa Viikari 44 Estimated H-index: Molecular and mutational analysis of a wheat thaumatin-like xylanase inhibitor TLXI.
Interlaboratory testing of methods for assay of xylanase activity
Sigrid Rombouts 1 Estimated H-index: Overexpression and partial characterization of a modified fungal xylanase in Escherichia coli. Production of cellulase-free xylanase by a thermotolerant Streptomyces sp. Charin Techapun 10 Estimated H-index: Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp.
Thermostabilization of Bacillus subtillis CCMI xylanases with trehalose study of deactivation kinetics. Hemicellulases in the bleaching of chemical pulps. Expression of a modified xylanase in yeast. Comparaison de la production de complexes enzymatiques par fermentation en milieu solide et par fermentation en milieu liquide.
Khushal Brijwani 1 Estimated H-index: Other Papers By First Author. Large-scale separation and production of engineered proteins, designed for facilitated recovery in detergent-based aqueous two-phase extraction systems process biochemistry.
Cloudberry Rubus chamaemorus cell culture with bioactive substances: Establishment and mass propagation for industrial use engineering in life sciences. Effect of pH on production of xylanase by Trichoderma reesei on xylan- and cellulose-based media applied microbiology and biotechnology. Michael BaileyJohanna Buchert. HimmelJohn O. Lowry Washington University in St.
LouisNira J. Rosebrough Washington University in St.
Are you looking for