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GLUT3 SPEC PDF

Compare Anti-Glucose Transporter GLUT3 antibody ab from Abcam on Be the first to write a review! Rabbit polyclonal to Glucose Transporter GLUT3. C5 and T4 cells showed the highest rate of GLUT-3 expression. These cells were ORL J Otorhinolaryngol Relat Spec. ;– glucose transporter 3 (GLUT3)2 is the major GLUT subtype in .. platelets without additional Ca2 at 20 °C in a F fluorescence spec-.

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On the other hand, estrogenic action on breast cancer progression and malignant transformation have been found to be related to the regulation of glucose transporter proteins [ 4 ]. Ductulogenic capacity and solid mass formation were evaluated as previously described [ 2830 ].

High correlations between primary tumours and loco-regional metastatic lymph nodes in non-small-cell lung cancer with respect to glucose transporter type 1-mediated 2-deoxy-2Ffluoro-d-glucose uptake. Especially within the last decade, expression patterns gluf3 GLUT isoforms mostly Glut1—5 have been studied in several tumor cell lines and tumor tissue specimens [ 4 ].

Differential regulation of glucose transporter expression by estrogen and progesterone in Ishikawa endometrial cancer cells. It has been well known that prolonged estrogenic exposure, such as early menarche, late menopause or hormone replacement therapy, have been considered main risk factors for the development of human breast cancer [ 2627 ]. Glut-1 antibodies induce growth arrest and apoptosis in human spex cell lines. Gillies R, Gatenby RA. In concordance with these findings, HIF-1 proteins and Glut1 expression have been extensively observed in aggressive phenotypes and glur3 breast tumors [ 1620 — 22 ].

However, when this cell line is subjected to carcinogenic exposure i. Interestingly, Glut12 co-expression has been observed as a second transporter with Glut1 in mammary epithelial cells during pregnancy and lactation [ 52 ]. spex

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They observed progressive increase of Glut-1 positive cell content in immunohistochemical sections according to tumor grade and stage. During pregnancy, sppec expression of Glut4 decreased progressively, whereas that of Glut1 increased [ 46 ].

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At the end of this period, the membranes of each chamber were fixed with Diff-Quick fixative and stained with DiffQuick solutions I and II Sigmacut out with a sharp scalpel, and mounted onto glass slides. Concordant with this suggestion, Rivenzon-Segal and co-workers [ 55 ] have found that differentiation of the breast cancer cell line T47D with retinoic acid led to a reduction in Glut-1 and to an increase speec cytoplasmic Glut-3 as well as decreased glycolysis.

Some oncogenes c-Myc, Ras and Src have been shown to activate HIF-1 system sspec well as glucose transporters [ 1819 ]. Similar results have been observed in normal and malignant breast tissues with respect to Glut12 expression.

Pathways of carcinogenesis and prevention in the human breast. Open in a separate window. GLUT expression patterns were investigated in an in vitro—in vivo progressive, estrogen-induced, mammary carcinogenesis model which consisted of four cell lines, with same genetic background.

Growth patterns in 3-D conditions as indicators of malignant transformation. Pereira3 J. J Mammary Gland Biol Neopl. Neeman M, Degani H. For example, malignant transformation of Rat1 fibroblasts with H-ras results in a substantial increase in glut1 mRNA levels in hypoxic conditions and thus, H-ras may specifically up-regulate Glut1 promoter [ 44 ].

Differential subcellular distribution of glucose transporters Glut and Glut9 in human cancer: Recently, high correlations between the primary tumors and metastatic lymph nodes with respect to Glut1 expression have been observed in several invasive tumors [ 4849 glu3. Cells which had crossed the membrane were collected and identified as C5.

J Natl Cancer Inst. Data are summarized in Table 2. For example, Suganuma et al. Therefore, estrogenic dependence is obvious in almost all steps of mammary carcinogenesis.

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In the present study, we attempted to identify the gene expression profiles of particular GLUTs in an isogenic group of cell lines in order to better understand the stages of specific alterations within estrogen-induced carcinogenesis.

However, the expression pattern of Glut3, particularly in preneoplastic and proliferative lesions, are unknown.

Regulation of glut1 mRNA by Hypoxia- inducible factor A family of glucose transporters GLUTs facilitates the movement of glucose across the plasma membranes in a tissue-specific manner, dependent on concentration gradient. Expression of glucose transporter-1 and -3 in the head and gout3 carcinoma—the correlation of the expression with the biological behaviors. In our previous studies, transforming potential of E2 on MCF10F was compared with chemical carcinogen benzo[a]pyrene BP by using the same in vitro model.

The quality and quantity of each sample were verified by spectrophotometry using NanoDrop 3. The concept of stem cell in mammary gland and its implication in morphogenesis, cancer and prevention.

Adaptation to hypoxia and acidosis in carcinogenesis and tumor progression.

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CA Cancer J Clin. Biochem Biophy Res Commun. Similar results can be obtained using Glut-3 antibodies or Glut-3 modulation methods in our model system.

A prospective study of endogenous estrogens and breast cancer glht3 postmenopausal women. T4-cancer cells were then isolated from these tumors [ 38 ]. For example, E2-transformed cells exhibited LOH in chromosomes 3 and 11, whereas BP did not induce genomic changes during the same period of time.

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