It is thus appropriate to modify as follows the existing Requirements for. Rabies Vaccine for Human Use. General considerations (page 58). Replace the. Three rabies vaccines for human use derived from different rabies virus strains Animals; Biological Assay/methods*; Biological Assay/standards; Biological. BIOLOGICAL ASSAY OF RABIES ANTISERUM: The potency of rabies antiserum is determined by comparing a lethal intracerebral dose of.
|Published (Last):||4 October 2007|
|PDF File Size:||4.95 Mb|
|ePub File Size:||16.81 Mb|
|Price:||Free* [*Free Regsitration Required]|
Transfer few cells of lacto bacillus leichmanii from a subculture onto 2 sterile tubes with 10ml culture medium. These two groups are used for titration of LD50 of challenge suspension. Thanks for shearing about this I thinks its very hopeful post and very important post for us.
Dissolve in following order L-cystine DL-trptophan dissolved in 1N — HCL and then Adenine-guanine uracil solution Xanthene solution Riboflavin — thiamine-biotin — nicotine acid solution P-amino benzoic acid — pyridoxine-pyridoxal- pyridoxamine solution. Oxytocin may be presented as a solid or as a solution in a solvent containing an appropriate antimicrobial preservative such as 0.
Rabies VACCINE – JSK NAGARAJAN |authorSTREAM
Oestrogen progesterone and prolactin — responsible for production of milk by memory gland but milk ejection require oxytocin. P otency of rabies vaccine is determined by comparing a lethal intra-cerebral dose of a rabies virus with the dose of the standard preparation of rabies vaccines necessary to give for same protection.
The test dose of the toxin is determined in relation to the Standard Preparation.
Virus vaccnie Virus replicated in muscle cells near site of bite for most of incubation time. Incubation time days. Rabies vaccine is a suspension of a suitable strain of fixed rabies grown in suitable approved cell culture and inactivated by a suitable method.
PHARMACOLOGY: Biological Assay of Diphtheria Antitoxin
For this purpose, a suitable preparation of diphtheria toxin is required to be used as a test toxin. Discard any values falling beyond the range. Severity of exposure Location of the bite The biting animal Bites on head and neck have shorter incubation time as short as 15 days because of rich peripheral nerve supply Encephalitic Form: You do not have the permission to view this presentation. Mice, 10gg -animal same sex.
The test toxin is allowed to stand for some months before being used for the assay of samples of antitoxin. Stay min protected from the light. Salt solution — A.
In selecting a toxin for use as the test toxin determine the following: Dose should be added at regular interval[minutes]. The proposed fifth International Standard PISRAV which was derived from the same virus strain as the present international standard preparation, the Pitman Moore PM strain, was found to be approximately twice as potent relative to the International Standard in immunogenicity assays as in antigenicity assays.
Any suitable strain rabies virus of known potency, such as the CVS strain may be used. Dephtheria anti-toxin is preparation containing antitoxic globulins that they have the power of specifically neutralizing the toxin formed by Cornebacterium diphtheria.
Sequential Steps of Rabies Transmission: Share to Twitter Share to Facebook. Automatically changes to Flash or non-Flash embed. Viral disease – cause acute encephalitis inflammation of the brain in warm-blooded animals. Observe the animals after 48hrs Mixture containing larger amount of toxin gives the larger reaction Mixture containing less amount of toxin gives the smaller reaction Slide 6: Similarly record the contraction of test preparation as standard.
The presentation is successfully added In Your Favorites.
There was a problem providing the content you requested
Rabies Vaccine Assay Test Animals: Transmission Almost all transmission is by bite 50 times greater risk than a scratch One human case may have been acquired in a laboratory transmitted by aerosol In wild animals: That is very interesting I love reading and I am always searching for informative information like this.
Inject the Virus 0. Standard preparation Test preparation To triplicate test tubes 0ml 0. Oxytocin facilitates the contraction of uterus.
Potency is determined by international reference Slide It is obtained by fractionation from boiassay serum or other mammals that have been immunized against tetanus toxin. The vaccine is prepared immediately before use vacccine reconstitution from the dried vaccine with a suitable sterile liquid.
The potency of rabies antiserum is determined by comparing a lethal intracerebral dose of rabies virus with the dose of standard preparation of rabies antiserum necessary to give same protection.